اثرات ضد دردي و ضد التهابي عصاره آبي - الكلي گياه بيلهر Dorema aucheri با استفاده از تست فرمالين و مدل كاراژينان در موش صحرايي نر

The Effect of Antinociceptive and Anti inflammatory of Hydro -Alcohol Extract of Dorema aucheri on FormalinTest and Carrageenan Model in Rats

Background and Objectives: Considering the importance of extracts of Umbelliferae herbs, the present study aimed to investigate the antinociceptive and anti- inflammatory effects of hydro alcoholic extract of Dorema aucheri. Material and Methods: In this experimental study, the experiments were performed on 80 male wistar rats weighting 190 to 200 grams, which were divided into 10 groups, each consisting of 8 rats. To evaluate the antinociceptive effects, the formalin induced pain-test was used and for assessing anti- inflammatory effects, the carrageenan induced hind paw edema model in rats was used. All animals were pre-treated with an oral dose of extracts (100, 200, 400 mg/kg). The control group received no drug and the sham group at only received distilled water. The first 0-5 min, and 16-60 min after formalin injection were considered as acute and chronic phases, respectively. The paw volume measured in the mercury from 0 to 2 hand 30 min after carrageenan injection. Statistical analysis of the data was performed using ANOVA and t-test where appropriate. Results: Results of this study showed that extracts of Dorema aucheri can significantly reduce the pain depending on the dose in acute phase (p<0.05). However, in chronic phase, the high dose of the extract could reduce the pain. These results also revealed that the highest dose of extract can reduce paw edema in carrageenan test. Conclusions: It seems that flavonoids can probably reduce intracellular calcium through inhibiting ofNMDA receptors. It also reduces activities of nitric oxide synthase enzyme and calcium dependent phospholipids A2, which results in antinociceptive effects. It may also be concluded that the anti-inflammatory effects of the extracts are through reduction of prostaglandins. Considering the above findings, Dorema aucheri as an antinociceptive and anti-inflammatory medicine could be introduced.