بررسي ميزان رنگ پذيري پروتئين p53 در اپي تليوم لكوپلاكيا و كارسينوم سلول سنگفرشي دهان با استفاده از تكنيك ايمنوهيستوشيمي

The Expression of P53 in Leukoplakia, Squamous Cell Carcinoma and Normal Oral Epithelium Using Immunohistochemical Staining Method

Background & Aims: Leukoplakia is the mostcommon premalignant or potentially malignant lesion ofthe oral mucosaand its potentiality for malignant transformation is unpredictable. The aim of the presentstudy wasto evaluate p53 expression in normal oral epithelium, leukoplakia and oralsquamouscell carcinoma. Methods: The standard Biotin streptavaidin peroxidase immunohistochemical staining method was used to study the expression of p53 on formalin fixed. paraffin embedded blocks of 8 cases of squamous cell carcinoma, 20 casesof leukoplakia and 10casesof normal oral epithelium. Results: There was no significant difference between immunostaining of leukoplakia and normal epithelium groupsin the expression ofP53. butthe distribution patterns of p53 was mainly localized in the basal layer in the groupof normal oral mucosa,while it extended into the suprabasal cell layer in leukoplakia group. P53 expression in squamouscell carcinomagroupwashigherthan other groups. Conclusion: Considering the findings the expression of p53 in suprabasal cell layers in leukoplakia might show poorclinical outcome and alterations of p53 might be an important factor in the development of oral cancer.