حذف فلزات سنگين مس و روي از پسمان هاي صنعتي يكي از كارخانجات صنعتي كرمان توسط باكتري هاي مقاوم جهش يافته جذب كننده فلز

Elimination of Copper and Zinc from IndustrialWastes by Mutated Bacteria

Background &Aims: Today, toxic effluents have created ecological and health problems in and around the industrial cities resulting in death of nearby living organisms. The aim of this research was to increase the elimination of copper and zinc from copper factory effluents in Kermanllran through mutation inducing in metal-resistant bacteria by using Acriflavine, Acridine orange and Ethidium bromide. Methods: A total of 20 strains of Pseudomonas spp. were isolated from water and soil of the factory and subjected to microbiological identification. Maximum Inhibitory Concentration (MlC) to Cu and Zn were determined by agar dilution method. Those strains with the highest M1C to these metals (5mM) were subjected to 400-3200mgIL of the above mutagenic agents. Afler determination of M1C those colonies which were capable to grow on 20mM copper were selected for atomic absorption spectroscopy. Results: According to the atomic absorption spectroscopy of dried biomass obtained from resistant strains after exposure to mutagenic agents, strains 6,7,8,9,10,13 & 16 showed the highest accumulation ofCU and Zn (IOmM for Cu & 20rnM for Zn). Strain 13 had the highest absorption ofCu (0. 35o/Jmg biomass) and strain 10showed the highest accumulation ofZn (0.33o/Jmg biomass). Conclusion: Elimination ofheavy metals by artificially mutated bacteria can be suggested as a cost effective solution to this environmental health issue.