مقايسه كارآيي دو روش HA BINDING و ZETA در جداسازي اسپرم هاي بالغ از لحاظ مورفولوژي و ساختار كروماتين طبيعي

The Comparison of HA Binding and Zeta Methods Efficiency in Selection of Sperm with Normal Morphology and Intact Chromatin

Sperm selection for ICSI is based on morphology and motility, but these parameters may not relevant chromatin integrity. One of the sperm selection methods for ICSI is based on sperm functional characteristics. This study was designed to compare two spenn selection methods (HA binding and Zeta method) for selection of spermatozoa with normal morphology and intact chromatin. Semen samples of 70 infertile couples referring to Isfahan Fertility and Infertility Center was assessed during this study. Semen analysis was carried out according to WHO criteria. Semen samples divided into 3 groups. A portion of neat semen considered as control group and remained of semen used for Zeta and HA binding procedures. Sperm morphology, protamine deficiency and DNA fragmentation were assessed by Papanicolaou sta ining, Chromomycin AJ (CMA3) staining, and SCD test , respectively in 3 group. Both HA binding assay and Zeta method are efficient to select sperm with normal morphology and lower protamine deficiency (P < 0.05). But in term of DNA fragmentation Zeta method appear to be more efficient to select sperm with low DNA fragmentation (P < 0.05) . Our results suggest that these sperm selection methods can select spermatozoa with normal morphology and protamine content for ICSJ. But Zeta method may be more efficient to select sperm with low DNA fragmentation. In patient with high DNA fragmentation , Zeta method can be useful to select spermatozoa for ICSJ.