ارتباط تعداد تكرار توالي CAG در ايزوفرم I ژن گيرنده آندروژن با سرطان و تومور خوش خيم پروستات

Association of CAG repeats of androgen receptor gene (Isoform I) with prostate cancer and benign prostatic hyperplasia

Background: An drogens th at act thr ough recept or s are requ ired for growt h a nd normal prost ate func tion. Androgen receptors (ARs) are important for the developm en t and progr ession of prosta te ca nce r. AR gene contains CAG repeats and it has been hypoth esized that sho r ter alle les of the CAG mi crosat ellite rep ea ls to be associated wit h an increas ed risk for prostate cance r. Ethn ic variations in CAG repeat len gth s may con tr ibute to varying prosta te cancer risks in differ ent populat ions. Methods: DNA from 110 pa tho logica lly-confirmed prostate cancer pa tients a nd 96 agematched men with benign prosta tic hyper plasia (SPH) were extrac ted and amp lified by nested polymerase chai n reaction (PCR). PCR prod uc ts were examined by elec tro phoresis and seq uenci ng. Sta tistica l analysis of the gathered data were ca rr ied out usin g SPSS software, version 13. Results: The mean number ofCAG repeats in prosta te cancer patients was sign ifica nt ly sma ller tha n those on BPH group ( 19.9 vs . 21.9 ; p