ارتباط تست هاي عملكرد اسپرم با نتايج روش تزريق داخل سيتوپلاسمي اسپرم (ICSI)

Correlation between the result of sperm function test and intracytoplasmic sperm injection (ICSI)

Background: Sperm function tests are series of tests that assess sperm function in fertilizing cggs at molecular levels. The correlation between sperm function tests and assisted reproductive technique s outcomes will give us a perspective on predi cting the outcomes of such tests. The aim of this study was to eva luate the relationship between sperm function tests such as sperm mitochondria, sperm chromatin structure, viability, etc with fertilization outcomes of ICSL Methods: Semen samples from 172 men attending Avicenna Infertility Clinic for intracytoplasmic sperm injection (ICSI) were collected. The remains of semen samples were used for extra sperm function tests after performance of routine semen analysis. Sperm chromatin integrity was evaluated by chrornomycin A3 staining for protamine deficiency, anil ine blue assay for abnormal persistence of histones, toluidine blue and acridine orange assays for DNA abnormality, rhodamine 123 for mitochondria and triple staining for assessing sperm acrosome status. Finally, the correlation between sperm characteristics studies and ART outcomes was examined. Results: There were no significant corre lations between the percentage of abnormal chromatin in sperm stained with AO, AB, TB and CMAJ and fertiliza tion outcomes following ICSJ. According to the results, sperm with abnormal mitochondrial and acrosorna l reactions have no significant correlation with fertili zation rates and quality of ferus. Conclusion: Considering the importance of sperm chromatin structure in protecting sperm genome, abnormal structures of chromatin could have negative influences on fertilization rates, although there were no significant correlations between sperm parameters and ICSI outcomes in this study. A possible reason accounting for the above result is the probable presence of interfering factors . Tests for sperm chromatin abnormalities are usually performed on rough semen samples containing large numbers of immotile, non-viable or degenerated sperm with abnormal chromatin. However, through pre-ICSI processing techniques and during ICSI, good quality spermatozoa are selected. Therefore, there is always the interference of selecting good quality sperm durin g ICSI programs, and embryologists try to select the best sperm with normal morphology and motility. In addition, there is still controversy over whether analytical techniques currently in use are capable of identifying the level of damage to spermatozoa. It seems that more studies are to be conducted to determine the effects of sperm chromatin damage on the outcome ofART.