بررسي تنوع ينتيكي برخي از توده هاي زيره كرمان با نشانگرهاي مولكولي RAPD

An Evaluation of Genetic Diversity in some Populations of Black Cummin (Bunium persicum) Using RAPD Markers

Black cumrnin, Bunium persicum (Boiss) is a native medicinal plant of Iran with a considerable distribution throughout the country. Regarding the necessity of a thorough study towards domestication of this valuable medicinal plant, in this research, genetic diversity of twenty four populations of black cummin was studied using RAPD markers. Fifteen RAPD primers of the highest level of reproducibility of template DNA and polymorphism among the banding pattern of the samples were selected for the study of populations. Two hundred and twenty eight bands were produced from among which one hundred and ninety six (86%) were polymorphic. Average polymorphism obtained for each RAPD primers was 13.97. Jaccardʹs similarity coefficient was used for an assessment of genetic similarities among populations. Estimates of genetic similarity based on the polymorphic bands ranged from 0.19 to 0.75 with an average of 0.53. Cluster analysis was performed based on similarity matrix and an application of UPGMA method. In the resultant dendrogram. maximum similarity was detected between Sirch and Jiroft-Darzin while minimum similarity observed between Bam, and Sistan & Baluchestan populations. The results showed a high level of genetic diversity among investigated populations. while also indicating that RAPD technique is an efficient tool for assessing genetic diversity in black cummin.