بررسي ميزان آلودگي گندم استان گلستان به زرالنون

NATURAL ZEARALENONE CONTAMINATION OF WHEAT FROM GOLESTAN PROVINCE. NORTHERN IRAN

Zearalenone contamination of 175 wheat samples produced in 2004 in Golestan province, northern Iran was determined. The 10-20 kg samples collected at different stages, including pre-harvest, hefore and after storage for 2 months in local silos. Samples were ground and sub-sampled with an analytical Romer mill. Each sample extract was cleaned-up using an immunoaffinity column. Zcaralenone levels were determined hy high-performance liquid chromatography (HPLC) with LTV detection at 275 nm. The linearity of the calibration curve in the range 12.5-750 nglml standard solutions of zearalcnonc was 0.997. The limit of detection lLOD) of the method was 30 ng/g. Mean recovery of the method was 85.5 % and 79 % at spiking levels of 100 and 200 ng/g, respectively. Fifteen samples ouL of 175 samples (8.6%) were contaminated. Mean level for contaminated samples was 72nglg and the range of contamination was 39-104 nglg. The proportion of contaminated samples was low and the levels were lower than the advisory levels for zcaralenone in wheat.