بررسي اثر دپرنيل بر تمايز سلول هاي استرومايي به سلول هاي عصبي توليدكننده دوپامين در موش صحرايي

Evaluating the effect of deprenyl on the differentiation of ratʹs bone marrow stromal cells into dopamine producing neurons

Background: Bone marrow strormal cells (BMSCs) can be used as a valuable cells source for auto­graft in clinical applications involving regeneration of the central nervous system. BMSCs are adult stem cells with the potential of differentiation into other types of cells. Research has proved the effectiveness of deprenyl in the treatment of neurodegenerative process of parkinson and also the trophic effect on the neural cells in vitro. Deprenyl has proven as inhibitor of monoamine oxidase B (MAO B), resulting in an enhancement of the dopamine level. In the present study the effects of deprenyl on BMSCs is evaluated. Materials and Methods: to evaluate the differentiated capability of BMSCs into the neuroid cells, the dopamine level and activity of tyrosine hydroxylase antibody were assessed by high performance liquid chromatography (HPLC) and immunocytochemistry, respectively. Also, the real time polymerase chain reaction (RT-PCR) was used for the study of the expression of brain derived neurotrophic factor (BDNF) gene in BMSCs. Data were analyzed by one way-ANOVA and post hoc Tukeyʹs test. Results: Molecular study indicated that the expression of the BDNF gene was increased in deprenyl- induced cells. Also, the immunocytochemical study of tyrosine hydroxylase and HPLC demonstrated that the deprenyl with concentration of 10"8 M has the potential of inducting the differentiation of BMSCs into dopaminergic cells and synthesis and secretion of dopamine.