تشخيص و رديابي ويروس Y سيب زميني به روش واكنش زنجيره اي پليمر از معكوس ( RT-PCR)

Detection of Potato Virus Y by Reverse Transcription Polymerase Chane Reaction (Rt-Pcr)

Potato Virus Y (PVY) a member of the largest family of viruses, Potyvirkiae, is capable of infecting a large range of plantsin several families eausing an economically devastating disease. In order to manage viral diseases, sensitive and accurate detection methods are required. In this slndy, RT-PCR was employed to detect Potato Virus Y in samples collected from fields inTehran province" DAS-ELISA test was initially carried out for all samples andthe infected samples were inoculated to test plants (using phosphate buffer). following total RNA extraction, with a pair of primers eomplimentary to the 5ʹ non- translated terminal and pi protein region, Rʹl:..PCRas well as imrnunoeapture RT-PCR were carried out. A 974bp fragment was amplified enabling us to recognize all known strainswitha high sensitivity. It was further shwn that,the plantswhich were kept at -20°C for upto 5 months did notshow anysignificant differences inPCRreaction.