كشت بافت ملج: اثر محيط كشت، نوع ريزنمونه و زمان برداشت آنها

Tissue Culture of Ulmus glabra Hudson: The Effects of Culture media, Explants and their Harvest Times

Elm (Ulmus glabra Hudson) species have been severely damaged by Dutch elm disease in recent decades. In this research node, leaf, and buds of U. glabra were sampled as explants for tissue culture. Percentage of explant survival was recorded following sterilization with sodium hypochlorite and mercuric chloride. The results showed that the best organs for tissue culture were buds. Survival percentage of the later fall bud explants treated with 2% sodium hypochlorite and 0.1% mercuric chloride was significantly different at 1% level when compared with the end winter buds. Shooting of buds performed in DKW basal medium with TDZ including IB A and DftW medium with BAP including IB A. We tested callus regeneration from shoot nodes in both MS and DKW media containing TDZ and BAP including NAA. Concentration of 0.44 mg l"1 TDZ and 3 mg I"ʹ BAP were affective in callus production. For increasing probability of callus regeneration, thay were subcultureed for 5 times during 5 mounths. The results showed that the best medium for regenerating plants from callus was DFCW containing 0.022 mg I"1 TDZ plus 0.05 mg T1 NAA.