اثر تعاملي تمرين هوازي و مصرف مكمل ويتامين E بر حذف MTDNA لكوسيتهاي خون انسان بعد از يك جلسه فعاليت وامانده ساز

The Interaction Effect of Aerobic Training and Vitamin E Supplementation on mtDNA Deletion in Human Leukocytes after Exhaustive Exercise

The purpose of this study was to determine the interaction effect of 8 weeks aerobic training and vitamin E supplementation on mtDNA deletion in human leucocytes after exhaustive exercise .Forty untrained, healthy and non-smoker students (age of 21.3±1.3 yrs, weight of 74.2±14.4 kg & BF% of 17.9±6.1) voluntarily participated in this study and randomly assigned to one of the 4 groups: (aerobic training+vitamin E), (aerobic training+placebo), (vitamin E), and (placebo). Subjects completed an incremental cycle ergometer test until exhaustion. The initial workload was 50W at 60 rpm for 5 min. Then, workload was increased progressively by 25W every 5 min until exhaustion while, the rpm was constant. Venous blood samples were collected I hour before and immediately after exhaustive exercise. Subjects of the groups I and 2 were influenced by independent variables during 8 weeks by 50-75% HRR (Carvonen method).Subjects of the groups 3 and 4 didnʹt perform any regular exercise during the period of study. In the groups of I and 3 subjects took a daily dose of 400 mg vitamin E. Subjects of the groups 2 and 4 took placebo containing sucrose. Blood sampling and performing exhaustive exercise were the same as prior to the study. The mtDNA deletion was analyzed using Multiplex Polymerase Chain Reaction (PCR) method. The results of Kruskal-Wallis and Sign test analysis demonstrated that there is a common deletion in human leukocytes mtDNA after exhaustive exercise bout (p<0.05). However, no significant differences were observed in mtDNA deletion after exhaustive exercise bout, after 8 weeks study. Therefore, discussion about effect of training on mtDNA deletion needs more studies.