عنوان مقاله :
مطالعه الگوي الكتروفورتيك پروتئيني Corynebacterium pseudotuberculosis جدا شده از موارد لنفادنيت پنيري در گوسفند و گاو
عنوان به زبان ديگر :
The study of protein electruphoretic pattern of the Corynebacterium pseudotuberculosis isolated from bovine and
ovine caseous lymphadenitis case
پديد آورندگان :
ممتاز، حسن نويسنده دانشكده دامپزشكي - دانشگاه آزاد اسلامي واحد شهركرد Momtaz, H , شاكريان، امير نويسنده دانشكده دامپزشكي - دانشگاه آزاد اسلامي واحد شهركرد Shakeria n, A
كليدواژه :
گاو , Biotype I and II nitrate positive and negative , ساختار پروتئيني , بيوتيپ 1و2 نيترات مثبت ومنفي , Protein structure and configuration , cattle , SDS-PAGE , SD S-PAGE , گوسفند , Sheep , Corynebacterium pseudotuberculosis
چكيده لاتين :
For stud ying protein elec trophoret ic pattern of Corynebacterium pse udotuberculosis , this study was co nduc ted on
16 bacteria isolat ed from ov ine caseous lymph adenitis, and they were compared wi th C. pseudo tuberculosis isolated
from cattle. All of microbi al spec imens und erwent ele ct rophoresis in SDS - PAGE method in the presence of low
molecul arl y we ight (LMWM) protein mark er on di screte sys tem ge l ʹ%5 - 10 polyacrylarnid, To tally 17 prot ein band s
with molecul ar we ight rang ing 25- 220 KDA were obse rved in the understudied bacteria. As a result, the protein s
3 1, 53, 64, and 68 KDA were iden tified in all stra ins of nitrate posit ive and neg ative bacteria , protein s 37>38 KDA
in biotype n . nitra te posi tive (iso lated from catt le) and prot eins 25, 35, 39, 40, 48 and 120 KDA in biotype I >nitrate
neg ati ve C. pseudotuberculosis (iso lated from sheep)
كلمات كليدي :
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