اثر اسمولاليته محلول سوكروز بر روي ماندگاري اسپرم شتر دوكوهانه

Effect of sucrose extender with different levels of osmolality on the viability of spermatozoa in bactrian camel (Camelus bactrianus)

This experiment was conducted at the bactrian camel research center, Jahadabad, Meshginshahr, Ardabil, during the rutting season to investigate the effect of sucrose extender with different levels of osmolality on the viability of bactrian camel sperm. Semen was collected from ten camel bulls with a modified bovine artificial vagina. Sucrose diluents with the adjusted pH to 6.9 and osmolalities of 292, 331, 356, 386 and 410 mosm/kg were made using respective 9, 10, 11, 12 and 13 percent sucrose media containing 20% egg yolk and antibiotics. The semen was homogenized, diluted at the rate of 1: 10, maintained at chilled condition (4°C) and evaluated immediately (time 0), 4, 12 and 24 hours after incubation at 4°C, for sperm progressive forward motility, plasma membrane integrity and live percentage of spermatozoa. Considering progressive forward motility and plasma membrane integrity of spermatozoa, 10 percent sucrose diluent was more suitable for preservation of semen at chilled condition for 4 hours compared to other osmolalities. The viability of bactrian camel spermatozoa declined drastically by 4 hours compared to time 0 (p<0.05). In conclusion, isotonic sucrose extender (330 mosm/kg i.e., 10 percent) with pH of 6.9 is better than 9, 11, 12 and 13 percent for shortterm preservation of bactrian camel semen at chilled storage condition.