مطالعه ايمونوهيستوشيمي تغييرات كلاژن نوع iv در غشاي پايه كلافه هاي گلومرولي در دوران جنيني و پس از تولد در موش نژاد Balb/c

Immunohistochmistry Study of Collagen IV Changes in Glomerular Basement Memebrane During Fetal and Postnatal Periods of Balb/c Mice

Purpose: In thi s investigat ion spec ific antibody type IV co llagen has been used in light micros copy to study development of BMG of the embryonic and postnatal mou se glomerular me sangium. Materials and Methods:20 female Balb/C mice were selec ted rand omly and were kept und er normal conditi on, finding vaginal plug was assumed as day zero of pregnancy. 12 pregnant mice were scarified by cervical dislocation in one of ges tationa l days 13- I8 and thei r fetu ses were fixed, serially sec tioned and immunohi stochemistry study for tracing of collagen type IV in BMG was carried out. The same pro ces ses were used for k idneys preparation on 5, 10,15 and 20 postnatal day s newborn s of 2 mothers for each day. Results: This study indicated that Collagen IV showed weak react ion on day 15 of gestation . The amount of collagen increased continuously until next days of fetal life and primary of 10 days postnatal in BMG. After this peri od, co11agen IV reacti on was not showed significant change in newborns . Conclusion: These data indicate that coll agen IV appear ju st during the glomerular vasculogenesis and because of co nt inuity with vasculature which is requ ired for glomerular endothelial cell differentiation , type IV collagen. is the maj or struc tur al protein in BMG implicated in these processes