بررسي اثر ضد رگ زايي عصاره آبي پيازچه موسير در مدل حلقه آئورت موش صحرايي

Anti..Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model

Objective: Angiogenesis is a key process in the promotion of cancer and its metastasis. Many natural health products inhibit angiogenesis. Because of the lack of molecular studies on anti-tumor and anti-angiogenic effects of shallot (Allium ascaJonicum); except a few clinical studies on other shallot properties , such as the anti-proliferative effect of shallot chloroformic extract on two tumor cell lines , the present study focuses on the anti-angiogenic effect of aqueous shallot extract using an aorta ring model. Materials and Methods: Aortic rings were obtained by cross-sectioning, at 1-2 mm intervals , the thoracic aorta of 4-B weeks old Wistar male rats and cultured them in a thin drop of type I collagen gel. After 3 days of CUlturing and first sprouting , the extract of A. ascaJonicum (from 25 to 800 ~g/ml ) was added to cultures . The results of anti-angiogenic activity were investigated by microscope. The cytotoxicity of extract at different doses on HUVECs was measured by trypan blue assay. Results: The results showed that the shallot extract has suitable anti-angiogenic effect in a range of 50 to BOO ~g lmt, but in 25 ~g/ml . the extract has no considerable effect. In addition , a tangible cytotoxic effect on endothelial cells at the above mentioned doses was observed . Conclusion: Our study showed that aqueous extract of A. escelonicum bulbs has noticeable anti-angiogenic activity without toxic effect on the cells in doses that ranged from 50-800 ~g l ml. Therefore, A. ascaJonicum can be a potential candidate for further investigations used in angiogenesis-related pathologic conditions.