كاهش بيان پروتئين هاي ميتوكندريايي در پروتئوم سيستم عصبي مركزي مدل حيواني بيماري مالتيپل اسكلروزيس

Central Nervous System Proteomics in Animal Model of Multiple Sclerosis Revealed Down-Regulation of Mithochondrial Proteins

Objective: Detection of central nervous system (CNS) molecular defects in an animal model of multiple sclerosis. Materials and Methods: Experimental autoimmune encephalomyelitis (EAE) was induced by a myelin oligodendrocyte glycoprotein. Protein expression profiles in the central nervous system between healthy clinical scores 1 and 3 of EAE were studied using a two dimensional electrophoresis based proteomics approach coupled with MALOI TOFITOF mass spectrometry. Results: We identified 8 mitochondrial proteins that were differentially expressed in CNS, all of them down-regulated in scores 1 and/or 3. Of these , 5 proteins belong to the mitochondrial respiratory chain including : NADH dehydrogenase (ubiquinone) Fe-S protein 8, cytochrome c oxidase Va, cytochrome c oxidase Vb, ATP5B. NADH dehydrogenase (ubiquinone) flavoprotein 2. We also observed down-regulation of three other mitochondrial proteins including : glutaredoxin 5, estradiol 17 beta-dehydrogenase Band isocitrate dehydrogenase. Conclusion: Down-regulation of mitochondrial proteins supported the hypothesis that hypoxia-like tissue injury in multiple sclerosis (MS) lesions may be due to mitochondrial impairment.