مطالعه تاثير فاكتورهاي رشد عصاره شش موش بالغ بر تمايز سلول هاي بنيادي بند ناف جنين به سلول هاي خوني در شرايط in vitro

The effect of adult mouse lung extracts growth factors in differentiation of umbilical cord stem cells into blood cells in vitro condition

Background: Stem cells (SCs) have great therapeutic indication due to their potency of self-renewal, multilineage differentiation, feasibility and safety for donors. In this study, adult mouse lung extracts containing hematopoietic growth factors were administered to umbilical cord, and evaluated the differentiation of umbilic al cord stem cells into erythroid and myeloid lineages. Materials and methods: In this basic and practical research, SCs were isolated from umbilical cord by enzyme digestion and cultu red in appropriate culture medium. Subjects were divided into four groups: Experimental groups I and 2 (El and E2) which were exposed to 50% and 70% concentration of lung extra ct for 7 days, respectively, sham (Sh) group which did not exposed to lung extra ct and cultured for 7 days , and control group (C). EI, E2 and Sh groups were incubated for 7 days. All groups were evaluated by alkal ine phosphatase detection kit for stem cells. Then, blood cells count and hematopoieti c growth factors were assessed. ANOVA was used for data analysis. Results: There were significant changes in E2 groups as compared with Sh and C groups, so that E2 group cells were differentiated into erythroid and myeloid lineages. Conclusion : Growth factors in lung extract could have stimulatory effe cts on umbili cal cord stem cell differentiation into blood cel1s.