ارزيابي تنوع ژنتيكي منابع ژرم پلاسم مركبات با استفاده از نشانگرهاي ريزماهواره (Microsatellite)

Evaluation of genetic diversity in citrus germless using microsatellite (SSR) markers

Genetic characterization and classification of germless are very important in breeding programmers. In this study 46 Citrus genotypes of Iran were classified based on data obtained from microsatellite marker banding pattern, using the Complete Cluster analysis. In this research nine pair of SSR primers generated a total of 54 alleles. The CAC33 with 9 alleles had the highest and TAA I7 with 4 alleles had the lowest number of alleles among the SSR markers employed. The number of heterozygote varieties varied from 21 in TAA27 and 43 in TAAI7 loci. The highest PIC value was related to TAAI5 (PIC=O.782) and the lowest was for TAA27 (PIC=OAI2). Cluster analysis divided the genotypes into 3 clusters. The genotype KC36 was very similar to KC4) and both of them were grouped in the same cluster and the least similarity with other types was related to the genotype KC41ʹ Also (Citrus maxima), (Citrus medica) and (Citrus reticulata) were placed in three groups. Based on results obtained from this experiment, it was inferred that among the studied SSR marker employed, TAA41, CACI5, CACI9, TAAI5, TAA33, CAC33 and TAAI revealed high distinct polymorphism. From the result of this study we suggest that these markers can be used for detecting genetic variability on Citrus germplasm.