تاييد جنسيت با استفاده از تكنيك PCR-RFLP در ژن ZFX/ZFY اسب نژاد عرب

Sex Verification of Arab Horse Using PCR-RFLP within ZFXlZFY Genes

In order to investigatie the possibility and accuracy of horse sex determination using PCRRFLP within ZFX/ZFY Genes, whole blood samples of 20 Arab horses (10 samples from . female and ]0 samples from male) were collected from Khozestan province. DNA was extracted using the modified salting-out procedure. Polymerase Chain Reaction (PCR), for amplification of 448 bp in coding region of ZFX/ZFY, was done using the primers, designed based on bovine ZFX/ZFY sequence. PCR products were digested by BSURI (Hael ll) restriction enzyme. PCR-RFJ.P genotypes consisted 01ʹ2 (403 and 45 bp) and 3 (448,403 and 45 bp) bands for female and male, respectively. Results indicated that sex determination accuracy in animals was 100%. The successful PCR for equine using bovine primers indicated high conservation and homology in ZFX/ZFY sequence between two species horse and cattle. In conclusion, this gene is a rcliable candidate gene for horse sex determination that can be used in sex determination of horse embryos sex.