اثرات انسولين و آنتي ونوم بر برخي پارامترهاي بيوشيميايي سرم خون خرگوش هاي تلقيح شده با سم عقرب همي اسكورپيوس لپتوروس

Effects of insulin and anti-venom administration on some biochemical parameters in rabbits envenomed by Hemiscorpius lepturus scorpion venom

This investigation was carried out to study the pathogenesis of Hem/scarp/us leptorus as one of the most dangerous scorpions of Khouzestan province, its cardiotoxicity and the role of insulin and antivenom therapy in rabbits, To this aim, 36 male New Zealand white rabbits with an average weight of 2000±200gr were divided randomly to four groups (A, B, C, and D) and each group was divided to three subgroups and injected with different doses of venom (1, 2 and 3 mg/kg, SC), These doses were administrated to all groups. Groups B and C were injected with insulin (0.25 IU /kg, 1M) and anti-venom (2 ml/rabbit, 1M) respectively. Group D was injected with both treatment at the same dose 20 minutes after envenomation. Blood samples were taken from jugular vein at the times before and 180 minutes after envenoming to measure the levels of CPK, LDH, glucose, Na, K, Mg and Ca. Statistical analysis showed significant increase of CPK after envenoming only in group A (P<0.05). The level of LDH increased significantly in all groups after envenomation (P<0.05). Administration of insulin prevented the elevation of glucose and CPK in most insulin injected subgroups. There was no significant changes for electrolytes. Thus, treatment with insulin, anti venom or both may prevent some biochemical disorders in scorpion envenoming