بررسي آناليز فيلوژني و شناسايي ويروس نيوكاسل به روش مولكولي در طيور استان چهارمحال و بختياري

Phylogenie analysis and molecular identification of Newcastle disease virus in fowls form in Chaharmahal-Bakhtiyari province

For molecular identification and phylogenie analysis of Newcastle disease virus in a chicken farm Chaharmahal-Bakhtiyari province, the seples collected of lung, spleen, trachea, brain and cecal toncil sample were collected hom suspected flocks. In RT-PCR method for identification of NDV, a primer amplified 356 bp products and a primer amplified 1349 bp products specific fix F protein genome for sequencing nucleotidcs \\ ere used. Results indicated that J00% of tissue sample tested with RT-PCR method were positive, Comparison of amino acids of isolated viruses in the cleavage site of F protein with standard velogenic and mild strain revealed that all the isolated viruses are velogenic and the sequencing of amino acids in isolated viruses were CiRRRQRRF. The phylogenie analysis revealed the genomic similarity ofisolated viruses with Russian and Italian strain.