مقايسه تاثير آنالوگ هورمون GnRH همراه با آنتي دوپامين دامپريدون (Ova-fact) به روش لينپه، با عصاره هيپوفيز ماهي كپور معمولي (CPE) بر شاخص هاي توليد مثلي ماهي بني (Barbus sharpeyi)

Comparision ofthe effectiveness GnRHa hormone (ova-fact) and carp pituitary extract on reproduction Indexes of Barbus sharpeyi

Barbus shunrcyi in family Cyprinidae is an endemic fish of the Khozstan province of Iran. The Iranian Fisherie Organization (Shilau produces up to 1-5 million Ir) (1-2 g b.w. I to restock the horal azcm and lagoon Shadgan population annuully.Thesc fish arc produced by artificial breeding using carp pituitary extract (CPE). The objective of this study was to assay the effectiveness of a gonadotropin releasing hormone analogue {D-Ala6, Iʹr09-NIʹt CinRH) in combination with the dopamine antagonist dornpcridonc (DOM) on spawning success, latency period, weight of stripped egg mass-weight of stripped egg rnaxstrcmnant ovaries, and fertilization success in Barbus sharpeyi, Seventy \\\0 fish were divided into 12 groups and injected intraperitoneally as follows: 5 mg.kg, b.w. of CPE as positive control, Gnkl la and nOM 30 Ilg/kg, b.w. double injection (10-9()ʹYO) 24 h apart. Propylene glycol injected fish were used as negative controls. The results showed that the GnRIIa and DOM in two injection lead to higher spawning success and weight ofstripncd egg mass.weight of stripped egg mass, and fertilization success and spaw ning rate and latency period in comparison with positive control (Iʹ <().OS), while no significant differences inhaching percent and embryo viability and masuring egg were found ( 1ʹ<(),05). ihcrcforc, it can be concluded that like many other cyprinids, dopamine inhibitory tone is activ C in Burbus sharpcv! and it is necessary to combine GnRHa with a dopamine antagonist for spa\\ ning induction.