بررسي و بهينه سازي استخراج اسيد آمينه ي فنيل آلانين به وسيله ي غشاي مايع امولسيوني آب ـ روغن ـ آب

Investigation and Optimization of Phenylalanine Extraction by W/O/W Emulsion Liquid Membrane

^ batch extraction of an essential amino acid, Phenylalanine, from an aqueous solution of different concentrations by an Emulsion Liquid Membrane (ELM) was developed using D2EHPA as a cationic carrier, Span 80 as the surfactant, paraffin and kerosene as the diluents, and HCl as the internal electrolyte. All effective parameters such as pH of initial aqueous external phase, electrolyte concentration in aqueous internal phase, carrier and surfactant concentration in emulsion, volume ratio of the organic to aqueous internal phase (Roi), volume ratio of the W/O emulsion to aqueous external phase (Rew) and time were examined and optimized using Taguchi method which was the first time of optimizing such number of factors by Taguchi method in ELM process. Appling Taguchi method to analyze the experimental results, we obtained the effects and contribution of each of the factors on the extraction efficiency. The results obtained from the experiments illustrated that with a stable emulsion by optimizing all the effective parameters, a considerable amount of phenylalanine can be extracted in a short time with an acceptable ratio of swelling and breakage.