القاي ايسكمي موضعي مغزي در موش صحرايي با مدل آمبوليك سكته ي مغزي

Local Brain Ischemia Inducing in Rat by Embolic Model of Stroke

In patients affected with stroke, most ischemic episodes (80-90%) occur due to occlusion of middle cerebral artery (MCA). Some of blood through femoral artery was withdrawn into a PE- 50 catheter and kept for 2 h in room temperature and 22 h at 4°C to allow old clot formation. Then the catheter was advanced 17 mm in the internal carotid artery until its tip was 1 to 2 mm away from MCA origin. 3-5 jil the preformed clot or 5 jliI saline (for sham- operated animals) were injected. Behavioral deficits and seizure activities were recorded at 2, 24, and 48 hours after clot injection. Then the rats were decapitated to remove the brains and prepare them for Tetrazoliume chloride (TTC) staining and analyzing. Received: 10.1.2008 Accepted: 16.3.2008 When 3 or 5 j_l1 clots were injected, infarct volume was 29.35± 1.26% and 16.15±94% respectively (p<0.05). Brain edema also was 8.6±1.65% and 3±1.74%, respectively (p<0.05). There was a significant correlation between infarct volume and brain edema (r=0.56, p<0.05). Behavioral deficit score at 48 hours after clot injection between two groups were significantly different (p<0.05). This model is very similar to thromboembolic stroke in human and prepares a reliable method for investigating the stroke mechanism. It would also be useful for studying thromboembolic agentsʹ effect on stroke and brain ischemic injury.