شناسايي گونه هاي Mesorhizobium گره زاي ريشه نخود ايراني جدا شده از خاكهاي استانهاي كرمانشاه و لرستان

Characterization of Mesorhizobium Strains Isolated from Chickpea Nodules from Kermanshah and Lorestan Provinces (Iran) Fields

Nodulated chickpea root samples were collected from Lorestan & Kermanshah provinces fields. Seventy two nodulating bacterial strains were then isolated using YMA medium. Infection tests were carried out and a total of 60 effective strains selected as chickpea nodulating strains. Through implementation of protein electrophoresis technique the strains were grouped while at least 30 strains selected for further characterization.The isolated strains were identified on the basis of morphological, physiological and biochemical tests. Protein electrophoretic patterns of the representative strains were compared with those of the type strains including Mesorhizobium ciceri USDA3383T, M mediterraneum ATCC51670T and M. loti ATCC33669T which were then characterized with their phenotypic features analyzed through SPSS software. Results indicated that the tested strains could be placed in three different clusters, representing three separate species of: Mesorhizobium mediterraneum, M. ciceri and Mesorhizobium sp.