ارزيابي اثر عصاره نام هيدرو الكلي گياه شنبليله بر ميزان اضطراب و خواب در موش سوري

Assessment of the Effect of Hydro*Alcoholic Extract of Trigonella Foeimm on Anxiety and Sleep in Mice

Background and Purpose: Previous studies suggest that the leaf of Trigonella foenum (TF) has analgesic, anti-inflammatory and probably modulatory effects on sleep and anxiety reactions. The present study is intended to determine the effects of hydro - alcoholic extract of TF on anxiety and sleeping time in mice. Methods and Materials: In this experimental study, 140 male albino mice (25-30 gr) were used in 14 groups (n=10 in each group). Elevated plus maze (EPM) was used for the assessment of anxiety, and experimental groups received hydro-alcoholic extracts of TF (50, 100, 200, 400 and 80 mg/kg) as IP injection; sham group received saline (10 mg/kg IP injection) 30 minutes prior to evaluation; the control group did not receive any injection. 25 minutes after injection, animals were put in a black box for 5 minutes in order to increase their activity; then, they were transferred to the EPM for anxiety evaluation by two factors: the time spent and frequency of entrance to the open arms of the EPM. In order to assess sleeping time, the Angel method (records of sleeping duration by physiograph) was used. 30 minutes before sleep assessment, TF extract and saline were used for experimental and sham groups; the control group received nothing as what we did in anxiety evaluation. Finally, ANOVA and Tukey tests were used for data analysis. Results: The findings indicated that the hydro-alcoholic extract of TF in doses of 100 and 200 mg/kg significantly increased the time spent and frequency of entrance to the open arms of EPM (p<0.05) in the experimental groups rather than in sham and control groups; this indicates a reduction of anxiety. Also, the TF extract in doses of 200 and 400 mg/kg significantly increased sleep duration (p<0.05) in comparison with the sham and control groups. Conclusion: The results indicated that the TF extract had an effective role in reducing anxiety reactions and increasing sleep