اثر دوره هاي كوتاه مدت ايسكمي بر كاهش آسيب ايسكميك بعدي در كليه موش صحرايي

The effect of short ischemic periods in reducing subsequent rat renal ischemic injury

Introduction: Using brief episodes of ischemia and reperfusion (IR) prior to a more sustained IR insult, i.e., ischemic preconditioning (IPC), can reduce IR injury of the heart, brain and many other tissues. The purpose of the present study was to investigate the effect of 2 min ischemic periods on subsequent rat renal IR injury. Methods: Renal IR injury was investigated in a right nephrectomized model in male rats. For this purpose, plasma creatinine (Cr) and urea, creatinine clearance, fractional excretion of sodium and histological injury score (Jablonski score; 0-4) were compared among the following groups; IR group (40min of renal ischemia followed by 24 h reperfusion), sham group (no IR) and IPC group (3 times of "2 min ischemia-5min reperfusion" before 40 min of renal ischemia followed by 24h reperfusion). Results: Necrosis score was significantly lower in the IPC compared with the IR group. Furthermore, rats with a Jablonski score of 4 were significantly less frequent in the IPC group compared to the IR group (11.1% vs. 75%). Plasma Cr and urea, creatinine clearance and fractional excretion of sodium were not significantly different between the IPC and IR groups. Rats with plasma urea levels higher than 190 mg/dl and also rats with fractional excretion of sodium beyond 2% were significantly less frequent in the IPC group compared to the IR group. Also Fractional excretion of sodium was not significantly different between IPC and sham groups. Conclusion: Using 3 times of "2 min ischemia-5 min reperfusion" before the injurious ischemic insult can reduce rat renal histological injury and partially attenuate functional renal injury.