ارزيابي تمايز مورفولوژيك كاج الدار و دو فرم طبيعي به وجود آمده از آن با استفاده از الگوي الكتروفورزي صفحه SDS، صفحات مورفولوژيك و برخي شاخص هاي فيزيولوژيك

Investigation of Morphological Identification in Pinus eldarica Medw. and its two Natural Generated Types Using lectrophoretic Pattern of SDS-PAGE, Morphological Traits and Some hysiological Parameters

In order to consideration and distinguishing of Mondell pine species (Pinus eldarica) and its two natural generated morphotypes, needles samples were collected from three years old seedlings in similar conditions and then extracted. Protein band patterns of the samples were studied and compared using electrophoresis method on polyacrylamide gel in the SDS-PAGE system. 12 morphological traits related to seedlings and mature individuals of the three pines, compared with each other. The amount of total protein also obtained using Bradford procedure and content of chlorophyll a and b measured by spectrophotometer with Harborne method. One dimensional electrophoresis of proteins showed perfectly similar isozyme patterns for each type (i.e. Mondell pine, Ball-shaped and Conical-shaped pines). Furthermore the amount of total protein and chlorophyll a and b contents of samples did not showed significant difference using ANOVA test. But 11 features of morphological measured traits were more in Mondell pine than the new generated pines. These results indicate the nonexistence of. biochemical differences among these pines in spite of obvious structural and morphological differences.