تاثير آفت كش ديازينون بر اووژنز و ساختار تخمدان موش هاي نژاد Balb/C

Effect of Diazinon as a pesticide on oogenesis and ovary structure of Balb/C mice strain

Diazinon is a contact organophosphorous pesticide which has a wide range of insecticidal application. In this study the effects of diazinon 60% EC (Emulsifiable concentrate) on oogenesis and ovary structure of mature and immature Balb/C mice were assessed. Olive oil was used as solvent. The lethal dose (LDso) was estimated as 68 mg/kgB.W. Mice were divided into three groups: control, sham and experimental. In experimental groups of mature (>50 days old) and immature (-30 days old), 40 mg/kgB.W of diazinon 600/0 EC were injected intraperitoneally once a day for 7 days. In sham groups, the same amount of olive oil were injected once a day for 7 days. 8 days after the first injection in mature mice and three weeks after the first injection in immature mice, the animals were sacrificed and ovaries were removed and studied macroscopically and microscopically. 5 micrometer serial sections of ovary were made and stained with Hematoxylin and Eosin. Then, results were analyzed statistically. In the experimental groups of mature and immature mice, no significant alteration in the weight of ovary and blood level of FSH and LH were observed when compared with control groups. Significant decreases (P<0.05 & P < 0.01 & P < 0.001) in the body weight, the number of primordial, primary and growing follicles, corpus luteum and its cells and the diameter of the Graffian follicles and their oocytes and nuclei, theca and granulosa layers and also the diameter of the corpus luteum and the blood levels of estrogen and progestin were observed respectively when compared with control groups. Also, significant increases (P<0.05 & P < 0.01 & P < 0.001) in the number of Graffian and atretic follicles and the diameter of ovaries were observed respectively when compared with control groups.