توليد و انجماد جنين گاو در آزمايشگاه

The Effect of vero Cell Co - culture on the Quality of In - vitro Produced Bovine Blastocysts after Vitrification

Introduction: The embryo quality can be assessed post freeze-thaw by various methods,the most common being in vetro survival after a period of culture Several culture system have been used for this purpose and most of them include somatic cell such as oviduct, granulosa and Buffalo rat liver cells as Co-culture support, The aim of this study was to assess the Vero cell Co-culture system on the quality of in - vitro produced bovine blastocysts after vitrification. Materials & Methods: After in vitro maturation and fertilization of Immature and SOFt medium bovine oocytes, presumptive zygotes were cultured in SOF2 for 7 days (39 c,5% Co ). Then 100 blastorcysts were selected and vitrifed (40% ethylene glycol, 18% ficol, 0.3 mol sacurose) After thawing the blastocysts were divided into two groups. One group was cultured in SOF? and the other group was cultured in SOF2+ Vero cell medium for 48 h. re-expanded blastocysts were selected, inner cell mass (ICM) from them were recovered by immunosurgery and stained cell with Hoechst. Results: The mean number of ICM in SOF2 and SOF2 +Vero were 12 and 15, respectively, the precentage of re-expanded blastocysts in SOF2and SOF2+ Vero were 42%,54% respectivety. There were significant differences between the number of ICM and percentage re-expanded blastocysts in the two groups. Conclusion: Vero cell Co-culture system can improve viability of in vitro produced bovine blastosysts after vitrifaction.