اثر محافظتي ويتامين E بر آپوپتوز ناشي از ديابت و وضعيت استرس اكسيداتيو در قلب موش صحرايي ديابتي

Protective Effect of Vitamin E on Diabetes Induced Apoptosis and Oxidative Stress in Rat Heart Tissue

Introduction: The purpose of this study was to determine and to assess the protective effect of vitamin E on cardiomyocyte apoptosis and oxidative stress status in the heart under hyperglycemic conditions, in vivo. Materias and Methods: Wistar male rats (n=16) at 6 months of age were made hyperglycemic by STZ. Same age, normal wistar rats (n=8) were used for comparison (controls). Diabetic rats were divided into two groups, the nontreated and those treated with vitamin E (300mg/kg/daily). Results: Diabetic rats exhibited severe apoptosis in cardiomyocytes. Also significant increases in lipid peroxidation as measured by 8- isoprostan, protein oxidation as measured by protein carbonyl content and superoxide dismutase were observed after 6 weeks. Catalase activity was shown to increase in controls compared to nontreated rats. A distinct elevation in the HbA1C, QT interval and a decline in the activity of catalase were also observed. Vitamin E treated rats shown significant decline in apoptosis, lipid peroxidation, protein carbonyl and QT interval compared to nontreated rats. Conclusion: Vitamin E decreased the incidence of apoptosis in cardiomyocytes, lipid peroxidation and improve antioxidant enzyme in the diabetic hearts of rats. Further research to confirm the findings is recommended.