بررسي رنگ پذيري ماركرهاي اي - كادهرين آلفا - كتنين در فوليكول دندان و آملوبلاستوما

Evaluation of the Staining Rate of E-cadherin and Alpha-catenin in Dental Follicle and Ameloblastoma

Background: Ameloblastoma is the most common odontogenic neoplasm, particularized by its local invasivenes and the destruction of the jawbone. The cause of local invasiveness of ameloblastoma has not clearly identified yet. To clarify the possible role of cell adhesion in local invasion of amelobastoma, expression of E-cadherin and alpha-catenin was examined in dental follicle and ameloblastoma. Materials and Methods: This research is a retrospective descriptive study and the specimens of 10 dental follicles and 33 Ameloblastomas (conventional amelobelastoma: 20 cases, recurrent ameloblastoma: 5 cases, malignant amelobastoma: 8 cases) were examined immunohistochemically using the streptavidin - biotin method. The data were analyzed using SPSS and statistical tests of Fisherʹs Exact and Mann-Whitney. P-value of 0.05 was considered as statistically significant. Results: Expressions of both markers were seen in all dental follicles. For ameloblastomas, expression of E-cadherin and alpha-catenin were detected in 66.7% and 69.7%, respectively. E-cadherin and alpha-catenin expression were lower in ameloblastomas than dental follicles. Rate of expression of both markers was lower in recurrent ameloblastomas and malignant amelobastomas, than conventional ameloblastomas. However, this difference was not statistically significant. Conclusion: Expression of both markers was different in dental follicle and ameloblastoma. Decreased expression of both markers relatively explains invasive biological behavior of ameloblastoma but due to its scarcity of the recurrences and malignancies, further investigations of more cases should be carried out.