مطالعه اثرات تزريق مرفين بر ساختمان ميكروسكوپيك جفت در موش

The study of morphine effects on microscopic structure of placenta in mouse

Background and Aim: Opioids consumption during pregnancy increases abortion. But the mechanisms that opioids increase abortion have been remained as a question. One of the mechanisms that may be caused to increase abortion is placental histological changes during pregnancy. The aim of this study was to determine histological changes after morphine consumption. Materials and Methods: Forty intact adult female Balb/c mice with two months-age, weighting 30-35 gr were used in this study. The animals were maintained at the animal house under controlled conditions (12 hrs light and dark cycle, 21°C and 50% relative humidity). Then they were mated overnight with 20 fertile males of the same strain. The day on which spermatozoa were found in vaginal smear was designated as embryonic day 0(E0). The pregnant mice were divided in to 4 groups (2 experimental and 2 control groups). Experimental group 1 received daily l0mg/kg morphine for 10 days and Experimental group 2 received daily l0mg/kg morphine for 15 days. In Control group, mice were injected l0mg/kg normal saline parallel to experimental group as a placebo. After this, in both control and experimental groups, mice were anesthetized and placentas were removed from the body. Then they were fixed in formalin for 48 hours. After routine histological processing and embedding in paraffin, all the specimens were cut into 5 Mm thickness sections and stained by Hematoxylin-Eosin. Then specimens were observed by light microscopy and photographs were taken. Results: In experimental group 1, two pregnant mice were died and one abortion was occurred. In experimental group 2, four mice were died and also two abortions were occurred. In control groups neither death nor abortion occurred. In experimental group 1 disruption of the placental villi was irregular and fibroblast cells were increased. In experimental group 2, intensive necrosis was seen. Conclusions: Our research showed that abortion, histological changes in placenta and significant neonate mortality rate and nervous system defect may be occurred after morphine consumptions.