اثر تحريك تخمك گذاري بر روي لانه گزيني و رشد جنين هاي موش

Evaluation the Effect of Super- Ovulation on Implantation and Fetal Growth in Mice.

Background and purpose: The objectives of this study were to evaluate the effect of super-ovulation with gonadotropins, on implantation and fetal growth after blastocysts transfer into the mice uterine. We compared the rate of endometrial acceptance after various number of blastocyst transfer into each of the uterine" s horn in mice. Materials and Methods: NMRI female mice 2-3 monthsʹ olds, were haphazardly divided into two control and experimental groups; and each was subsequently was divided into two groups,ʹ donor and recipient. All of donors and experimental recipients were super-ovulated with PMSG and hCG. 5 and 10 obtained blastocysts were then respectively transferred into left and right uterine horns of pseudo-pregnant recipients. All recipients were killed on the 14th. day of pregnancy and were evaluated from the rate of implantation and fetal weights. Results: No significant statistical differences were found in the grown fetal rate in super-ovulated recipients (6.1%), as compared with control recipients (7.3%). The grown fetal rate in the left horn of uterine were (13% in control recipients and 11.4% in super-ovulated recipients) was significantly higher than in the right horn (4.5% in control recipients and 3.5% in super- ovulated recipients). Furthermore, no significant statistical differences were found in the rate of not grown fetuses in super-ovulated recipients (10.4%), as compared with control recipients (12.3%). Moreover, no significant statistical differences were found in the mean weight of fetuses obtained from super ovulated- recipients as compared with that of fetuses obtained from the control recipients. Conclusion: No significant effect was observed with ovarian stimulation on the implantation rate and fetal growth. The capacity of uterine acceptance was decreased with added embryos.