بررسي اثر فاكتور مهار كننده لوسمي در غلظت هاي متفاوت بر حركت و بقاي اسپرمهاي مردان نابارور مبتلا به آستنواسپرمي

The Effect of Different Concentration of Human Recombinant of Leukemia Ingibitory Factor on Sperms of Asthenospermic Men

Purpose: Knowing that there is no report on the effect of LIF on low motility sperms of asthenospermic men we decided to determine the fact effect of this factor on low motility sperms. Materials and Methods: In this investigation the sperm sample of 15 infertile men, who have already referred to IVF department of Imam Khomeini hospital, were collected and then prepared them by successive stages for culturing in Hamʹs F10+FCS 10% medium. Then, the samples were put in the medium containing various concentrations (0 ng/ml, 3 ng/ml, 5 ng/ml, 10 ng/ml, 50 ng/ml) of LIF at 37°C and under 5% of Co2 in air for up to 48 hours. Sperm motion characteristics were measured using Makler chamber. Also, Sperm survival was determined by hyposmotic swelling test. Collected data with ANOVA method by means of SPSS software were analyzed. Results: Sperm Motility and survival were not significantly enhanced up to 6 hours exposure to different concentration of LIF, Howevere sperm progressive motility were significantly higher after 24h exposure to 10 ng/ml of LIF. P<0.05. The survival rate of sperm was significantly prolonged in that time exposure to 10 ng/ml of LIF P<0.05. progressive motility of sperms and survival rate were significantly higher after 48h exposure to 50 ng/ml of LIF. P<0.05. Conclusion: It can be concluded that the effect of Leukemia Inhibitory Factor on sperm motility and survival were concentration-dependent and that LIF increased sperm motility and survival in vitro after at least 24 hours of hours of incubation. We could not find significantly differences in shorter incubation period.