وجود عفونت ويروس پاپيلوماي انساني در بافتهاي سرطاني پستاني و ارتباط آن با شاخص هاي باليني

Presence of Human Papilloma Virus Sequences in Breast Cancer Tissues and Association with Histopathological Features

Introduction: Several studies confirmed oncogenic roles of viral agent in Development of breast cancer. Human papilloma viruses especially high risk types (16, 18), are demonstrated in epithelial malignancy of genital and extra genital organs. This study was done to examine the presence of high and Low risk groups of human papiloma virus (HPV) in breast cancer tissues and their correlation with histopathological characteristics. Methods: Fifty specimens of breast cancer tissues and 29 samples of non-malignant breast tissues were studied for the presence of DNA of HPV of the low risk groups(l,6) and high risk groups( 16,18) by polymerase chain reaction (PGR) with HPV consensus primers . Results: Papilloma virus DNA was detected in 24 (48%) samples of breast carcinoma that 26% of them harbored high-risk DNA sequences of HPV in their tumors (13 patients) and 8 patients (%16) had low risk HPV infection .Three breast cancer tissues were doubly infected by high and low risk HPV (6%). All of none malignant mammary tissues were free of HPV infection (high and Low risk). In this study, there was no correlation between the presence of DNA of HPV and prognostic factors of breast cancer such as age (/M).448), axillary lymphadenopathy (P=0.749), histopathologic grade of tumor (F=0.946), tumor size (/M).946) and laterality of the tumor. Conclusion: The presence of high and low risk DNA sequences of HPV in the breast cancer tissues was verified in this study and a probability association in the acceleration and development of breast malignancy were confirmed.