بررسي ارتباط بين چند شكلي ژني (A→C) 1188+ ژن IL-12 با سطح سرمي سيتوكين IL-12 در بيماران مبتلا به عفونت نهفته هپاتيت B

Evaluation the Relationship between Alleles of +1188 in Region of IL-12 with Serum Level of Cytokine in Patients with Occult HBV Infection

Introduction: Occult hepatitis B infection (OBI) is a form of hepatitis, which in despite of absence of detectable HBsAg, HBV-DNA is present in peripheral blood of patients. Objective: Evaluation the relationship between alleles of+1188 in region of IL-12 with serum level of cytokine in patients with occult HBV infection Materials and Methods: In this study, the plasma samples of 3700 blood donors were tested for HBsAg and anti-HBc by ELISA. The HBsAg negative ve and anti-HBc positive samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples assigned as OBI cases and PCR-SSP and ELISA were performed to examine the polymorphisms in region of (+1188 and serum level of IL-12) respectively. Results: The results showed that there is a significant difference in CC allele of +1188 region of IL-12 in two groups and no difference in the other evaluated genes. There is not any significant difference in serum level of IL-12 between OBI patients and controls. Our results also showed that there isnʹt any significant statistically relation between alleles of+1188 region of IL-12 with serum level of cytokine. Conclusion: According to the results of this study it could be concluded that OBI patients unable to produce enough quantity of IL-12 and it may be related to different IL-12 gene. CC allele was associated with OBI, hence, it seems that +1188 region of IL-12 gene has an important role in expression of IL-12 gene. Evaluation of relation between polymorphisms in +1188 region of IL-12 gene and its expression. In vitro and under mitogene affect can useful because no association was seen between serum level of IL-12 and alleles of this region