مكان يابي QTLهاي كنترل كننده عملكرد در جمعيت 2F دو رقم برنج ايراني

Mapping QTLs controlling yield in two Iranian rice cultivars-F2 populations

In this study, a linkage map of 117 AFLP markers was constructed in an F2 population derived from a cross between two Iranian rice varieties, Domsephid and Gerdeh. This map used for identification of the QTLs controlling yield in F2. Average grain yield in parents Gerdeh and Domsephid were 43.25 and 31.55 g/plant respectively, and t-test showed significant difference (P<0.01) between two parents. Genotypes were determined for F2 individuals using 117 AFLP markers. The linkage map of the population was generated with Map Manager V2.6.5. Map length was 1212 cM with an average interval size of 11.88 cM. QTLs analysis were carried out to determine the relationship between marker genotypes and trait values in F2 individuals using simple interval mapping (SIM) and composite interval mapping (CIM). In F2 population, one QTL associated with yield was determined on linkage group 2. Phenotypic variation associated with this QTL and its additive effect was 8.3% and 4.74 gr, respectively. In this QTL, alleles from Gerdeh increased grain yield.