اثر تزريق درون بطن-مغزي عصاره الكلي سرشاخه هاي گلدار علف چاي (Hypericum perforatum)در بروز رفتار ناشي از احساس ترس در حضور پنتيلن تترازول (PTZ) در رت نر بالغ

Effects of Intracerebroventricular Injection of Alcoholic Extract of Hypericum Perforatum on Fear Behavior in Presence Pentylenetetrazol (PTZ) in Adult Male Rat

Introduction: Fear is a biologic response that protects animals from acute danger. Fear and anxiety are important subjects in psychological science. Different mechanisms and drugs have been introduced for their control and therapy. Herbal drugs have mild effects on fear and are more sedative than chemical drugs. Therefore, the researchers tried to test them on medical treatments, especially on those associated with panic and anxiety. Materials & Methods: In this research, the effect of intracerebroventricular (I. C. V) microinjection of Hypericum perforatum on fear was studied. Animals were divided into 5 groups: saline, pentylenetetrazole (PTZ) 20mg/kg as positive control (i.p) and two groups that received 10, 20jig/rat alcoholic extraction of H perforatum (ICV) respectively, and one group that received 10|ig/rat alcoholic extraction of H. perforatum (ICV) and PTZ (i.p). A cannula was placed into lateral ventricle by stereotaxic apparatus. After the recovery period, elevate plus maze evaluation of the fear. Findings: Open arm entries percentage (%OAE) and open arm time percentage (%OAT) were evaluated. The results showed that 10 fig/rat and 20 jig/rat doses increased %OAE and %OAT significantly. (P<0001) Discussion & Conclusion: We concluded that alcoholic extraction of H. perforatum is an effective drug in fear reduction. It probably acts on serotonergic and gabaergic system and decreases their re-uptakes and reduces the fear caused by PTZ.