بررسي تنوع ژنتيكي توده هاي تره ايراني ( Allium ampeloprasum)با استفاده از نشانگر رپيد ( RAPD)

The study of genetic diversity of Tareh Irani (Allium ampeloprasum) populations using RAPD markers

RAPD analysis offers a quick and comparatively cheap approach for the detection of genetic differences, since it has extensively been used in studying genetic diversity in different plants. This technique was employed to assess genetic diversity of 28 local populations of Tareh Irani (Allium ampeloprasum) and to examine their relationship with kurrat (Allium ampeloprasum Kurrat Group) and leek (Allium ampeloprasum Leek Group). Nine of 320 random 10-mer primers revealed scorable polymorphism between populations of Tareh Irani and it would be further evaluated for use in genetic mapping. Genetic distances between each of these plants were calculated and cluster analysis was used to generate a dendrogram showing phylogenetic relationship between them. Leek and Kurrat felt in two distinct groups, separated from Tareh Irani populations. Tareh Irani populations also were separated from each other in 6 groups. Most of them were belonged to 6th group indicating the low genetic distance between them. Ahvazl9, Zabol26 and Arak58 populations were located individually in three distinct groups, having higher genetic distances with other populations. The results were in agreement with previous studies.