چكيده لاتين :
Aims. In the present study, the antinocieceptive activity of saffron (Crocus sativus) water-extract in female N-MARI mice (20-25 g) investigated.
Materials & Methods. Formalin test applied for pain assesment. 30 minutes before 2% formalin solution (2 (il) injection in posterior plantar angle, the drugs administered to the animals. Then the animals evaluated for pain assesment for 60 min in a 5 min interwalls manner.
Results. Morphine (1,5 and 10 mg/kg) administeration induced antinocieception in both acute and chronic phases of formalin test in a dose-dependent manner. Extract (10,50 and 100 mg/kg) also induced analgesia in chronic but not acute phase of formalin test. Naloxone (4 mg/kg) administration had no effect on the antinocieceptive activity of the extract. Dextromethorphane (20 mg/kg), on the other hand, enhances the extract action in acute phase as well as chronic phase. In addition, L-NAME (10 mg/kg) administration potentiate the extract effect. Co-administration of ineffective dose of indomethacine (2.5 mg/kg) with the extract (2.5 mg/kg) had no influence on the extract antinocieception effect.
Conclusion. Water extract of C. sativus exerts its effect on the chronic phase of formalin test, possiblly via inhibition of NMD A glutamatergic as well as nitric oxide system.
