بررسي بيان ژن هاي قابل القاء توسط تنش شوري طولاني مدت در دو رقم حساس و متحمل گندم

Evaluation of inducible gene expressions under long term salinity stress in tow susceptible and tolerant cultivares of wheat

In the present work expression of 61 candidate genes which are induced by salinity stress in mahooti and Chinese spring cultivars were evaluated and compared under 0 and 250 mM NaCl. In order to the study of candidate genes expression after preparing PCR products for each gene, were blotted on the membrane and after that hybridization were performed using cDNA probes in nonstressed and stressed plants. By means of TotalLab software, darkness of spots on the x-ray film were quantified. Results indicated that positive and significant correlation between biological replication exists. From 61 candidate genes, 21 and 5 candidate genes significantly were upregulated in mahooti (34%) and Chinese spring (6%), respectively. Under salinity and non salinity stress 52 and 27 percent of studied candidate genes in mahooti respect to Chinese spring cultivar considerably had higher expression level, respectively. The greatest increase and decrease in expression ratio under salinity stress to control condition was observed in TVP and HKT1, in mahooti and Chinese spring cultivars, correspondingly. The results of this study show that the different pattern of gene expression can be associated with salinity tolerance in mahooti cultivar