كليدواژه :
مقاومت آنتي بيوتيكي , بتالاكتاماز , اشريشياكلي , ايزوله هاي ادراري
چكيده لاتين :
Increased spread of antibiotic resistance and treatment failure among Escherichia coli isolates, most common agent of urinary tract infection, can be related to the increasing prevalence of expanded spectrum (3-lactamases (ESBL) clinical isolates. The present study was conducted to examine the antimicrobial resistance of urinary isolates of E. coli in Mashad and to detect the ESBL producing strains among them.
Methods and Materials: In this descriptive study, 109 isolated E. coli were identified using differential biochemical experiments, from urine samples of hospitalized patients in Mashad hospitals (Ghaem and 17-Shahrivar Hospitals). The antibiotic susceptibility was examined by disc diffusion method according to Kirby-Bauer standards. Detection of ESBL producers were done by double disc method. Data were analyzed by Statistica software using Chi-square. Findings with p<0.05 were considered as significant differences.
Results: It was observed that %55.05, %34.86, %21.10, %12.84, %2.75 and %1.83 of clinical isolates were resistant to co-trimoxazole, nalidixic acid, ciprofloxacin, gentamicin, polymyxin and nitrofurantoin respectively. The highest and the lowest resistance were against co-trimoxazole and imipenem respectively. Twenty two clinical isolates of E. coli had multi-drug resistance to ciprofloxacin, nalidixic acid and co-trimoxazole. Double disc test was positive for 35 (32.11%) of 109 isolated E. coli bacteria and a high rate of associated resistance to co-trimoxazole, quinolones, gentamicin and polymyxin was found in ESBL producers (p<0.05).
Conclusion: As the results of the study indicated, ESBL producers have high prevalence among the E. coli isolates in this
population. (Quarterly Journal of Sabzevar University of Medical Sciences, Volume 16, Number 4, pp.227-232).
