بررسي ميزان القا ژن gamma گلوبين با غلظت هاي مختلف هيدروكسي اوره در رده سلولي k562 با هدف درمان بتا تالاسمي

Determination of y-globin induction levels by different concentrations of hydroxyurea in K562 cell lines for p thalassemia treatment

Background: Hydroxyurea (HU) is currently used for (3 thalassemia treatment through induction of fetal y-globin. In this study, effects of different concentrations of hydroxyurea on induction of y-globin gene in K562 cells, and inhibitory effect of siRNA against candidate gene which may be involved in HU mediated y-globin induction were assessed. Materials and Methods: In this experimental study, K562 cells were treated by different concentrations of HU (0, 50, 100 and 200 |liM). siRNA against candidate gene in HU mediated y- globin gene induction was transfected to K562 cells using lipofectamine 2000. The level of y-globin gene induction and inhibition were determined by quantitative real-time PCR. Results: There were 1.75-, 2.45- and 2.55- fold inductions in y- globin gene using 50, 100 and 200|iM HU, respectively. There has been 79.5% down regulation on candidate gene in siRNA transfected K562 cells. Conclusion: This study showed that y-globin induction in 100|nM HU is similar to 200 |iM HU treated K562 cells. There was also efficient inhibitory effect on candidate gene which may be involved in HU mediated y-globin induction