استفاده از ويروس هاي نوتركيب مشتق از HIV-1 براي ترانسداكشن سلول هاي HT1080 با هدف ژن درماني بيماران بتا- تالاسمي

HIV type 1-based particles effect in transduction of HT1080 cell line: A technique in p-thalassemia gene therapy

Background: One of the most effective methods in the treatment of p-thalassemia is gene therapy by viral vectors. The aim of this study was to design a recombinant lentivirus containing mini LCR and p- globin gene for transferring normal p-globin gene into hematopoetic stem cells. Materials and methods: In this basic-applied study, each segment was cloned into a lenti transfer vector and confirmed by restriction digestion and sequencing. Transfer vector and three packaging plasmids were cotransfected into 293T packaging cells using lipofectamine 2000. Harvested viruses were confirmed by RT PCR on extracted RNA of these recombinant lentiviruses. Results: The titer of lentiviral stock was determined in a HT1080 cell line. Transduction of target cells was increased by polybrene until 2 fold. Transduced HT1080 colonies remained after 2-week antibiotic selection. The remained transduced HT1080 colonies were expanded and DNA was extracted. PCR evaluated random integration of construct into the genome in this gene transfer technique. Optimum MOI for HT1080 cell line was determined Conclusion: Lenti viruses can be used for effective and permanent gene transfering in mammalian cells such as hematopoetic stem cells in order to accomplish gene therapy of genetic diseases like beta thalassemia and cancers.