تاثير مورفين خوراكي بر تكوين بطن هاي جانبي و شبكه كروييد در جنين موش صحرايي

Effect of oral morphine consumption on lateral ventricles and chroid plexus in rat embryo

Aims. Previous studies have shown that morphine consumption during pregnancy may delay embryo development. The present study focused on the effects of maternal morphine consumption on choroids plexus development in Wistar rats. Materials & Methods. Wistar rats (170-200g) were used throughout. The experimental groups after pregnancy received 0.05mg/ml of morphine in their tap water while the control group received water. On 17th day of pregnancy, the pregnant animals were killed by chloroform and the embryos were removed surgically and fixed in formalin 10% for 4 weeks. Then, tissue processing, sectioning and staining with hematoxylin and eosin were applied for the embryos. The sections were examined for choroids plexus and ventricle development by light microscope and MOTIC software. Results. Severe reductions of the third as well as lateral ventricles were observed in the experimental group. In addition, an increase in the choroids plexus area in the experimental group regarding to controls was identified. Conclusion. Oral morphine consumption causes a decrease in the third and lateral ventricles and an increase in choroids plexus area. This defect may cause behavioral changes observed in the F1 generation from addicted pregnant animals.