بررسي اثر روش استخراج بر تركيب شيميايي و فعاليت ضدميكروبي اسانس گياه زنيان (Carum copticum)

Studies on the Effect of Extraction Method on Chemical Composition and Antimicrobial Activity of Carum copticum Essential Oil

Essential oil of Carum copticum cultivated in Iran was obtained by hydrodistillation (HD) and microwave free solvent (SFME). Oils were analyzed by GC/MS. Identification of the Components were preformed by comparison of the relative retention indices and mass spectra with authentic reference [1]. Eleven compounds (99.31%) were identified in the oil which was isolated by hydrodistillation. The major compound of the oil of C.copticum were y-terpinene (37.40%), p-cymene (28%) ,thymol (24.96%), p-pinene (5.78 %.), myrcene (1.31%). Eleven compounds (96.91%) were characterized in the oil which was obtained by free-solvent microwave extraction. The major compounds of the oil were thymol (35.28), y- terpinene (32.49%), /?-cymene (23.51%), p-pinene (3.47%), carvacrol (2.46).Antimicrobial activity of the essential oils were examined against Escherichia coli, using agar dilution method and minimum inhibitory concentration (MIC) was determined at 500ppm.