بررسي اثر ضدويروسي عصاره آبي و الكلي گزنه دو پايه (Urtica dioica L) در حذف عامل ويروسي ايجادكننده موزاييك رز در محيط كشت

Study on the antiviral effect of aquatic and alcoholic extracts of Urtica dioica L. on rose mosaic viral disease in vitro culture

Stinging nettle has been used for treating several human chronic diseases. This research focused on the use of Urtica dioica L. (stinging nettle) aquatic and alcoholic extracts for the production of mosaic virus-free rose plants by explant culture from infected parent material. Toward this aim a survey was conducted to detect the viruses infecting Rosa species in main rose growing plantations of Tehran province and its suburbs. Using Enzyme-Linked Immunosorbent (DAS-ELISA) and Dot-Blot (DIBA) serological methods and polyclonal antibodies, 530 rose samples were tested for the presence of Prunus necrotic ringspot virus and Arabis mosaic virus. Transplant medium culture in MS medium was performed for a 20 infected rose transplants which were infected with ArMV and PNRSV viruses. Dried leaves and roots of nettle plants were extracted with water and 95% ethanol. Aquatic and alcoholic (ethanol) U. dioica extracts inoculated with three different concentrations (5, 2, 0.5 mg/ml) (extract/ml medium) into MS media. Rose transplants were serologically tested for the presence of viruses 30 days after treating. Results indicated that the aquatic extract of nettle plants at the concentration of 5 and 2 mg/ml and ethanol extract at the concentration of 0.5 and 2 (mg/ml) are effective in eliminating 90% of PNRSV and 42% of ArMV respectively. According to the results of this study U. dioica aquatic and alcoholic extracts are able to adversely affect the rose mosaic virus replication. They may be used as an herbal source as viricidal preparations against rose mosaic disease caused by ArMV and PNRSV.