آناليز فيلوژنيك تعدادي از سويه هاي مايكوپلاسما سينوويه جدا شده از مرغداري هاي صنعتي استان مازندران

The phylogenetic analysis of some Mycoplasma synoviae isolates from Mazandran province poultry flocks

Mycoplasma synoviae (MS) is one of the important pathogens in chicken and turkey which causes great economic losses in poultry industry. The aim of this study was DNA sequence analysis of some Mycoplasma synoviae isolates from Mazandran province poultry flocks and compare them with MS from other countries. For this purpose, 32 samples were collected from choanal cleft, trachea and air sac of flocks with respiratory symptom and cultured in specific PPLO medium. Ten out of 32 samples were positive in culture and reacted with specific antisera. The 10 positive samples were subsequently used for molecular study. The PCR products containing 16s rRNA genome were generated with specific primers and then sequenced. Multiple alignment and phylogenic analysis of DNA sequences were performed with DNASTAR program. Phylogenic analysis of MS isolated from Mazandran province and 21 MS from all of the world revealed low homology among most of them. However some of the Mazandran isolates showed high homology with Ms isolated from Brasilia. Overall, these results revealed the different molecular structure and heterogeneity among MS isolated from Mazandran province in this study.